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The effect of osteocalcin on testis morphometry in azoospermic Syrian male NMRI
Mahsa Yaghobinejad Stuent1 , Somaye Toolee Stuent1 , Heidar Solhjoo Stuent1 , Aliasghar Bashghareh Stuent1 , Aliasghar Nohesara Graduated1 , Tayebe Rastegar Professor 2
1- دانشگاه علوم پزشکی تهران
2- دانشگاه علوم پزشکی تهران , trastegar@sina.tums.ac.ir
Abstract:   (111 Views)
Aim: The aim of this study was to evaluat the protective effect of osteocalcin on testis tissue  in adult azoospemic mice.
Method and materials: Twenty five Adult male mice were divided into five groups: the control group doesent received any interference, sham  group(received DMSO and after 5weeks they received PBS for 30 days), and busulfan group received a single dose of 40mg/kg busulfan intra-peritoneally, and, and the  busulfan- osteocalcin group received 3ng/g/d osteocalcin, daily for one month, 5 weeks after a single dose of busulfan. Finally after removal of the testis, tissues were fixed in bouin solution and stained by H&E. the sections were evaluated, and morphometric tests were done.
Results: No significant reduction was found in body weights of the groups, However left testis weight in osteocalcin group showed significant raise in comparison with azoospermic group(p˂0/05). More ever, significant increase in seminiferous tubule diameter was observed in the osteocalcin group in comparison with azoospermic group(p˂0/05). Testicular epithelium height was significantly increased in the osteocalcin group in comparison with azoospermic group (p˂0/05). There weren’t any significant changes in sertoli, leidyg, myoid counting among all the groups. the total number of spermatocyte, round spermatid, elongated spermatid and spermatogonia cells showed significant increase in osteocalcin group in comparison with busulfan and busulfan-PBS groups (p˂0/05).
Conclusion: osteocalcin can improve the cytotoxic effect of busulfan on spermatogenic cells, also can improve spermatogenesis.
 
Keywords: osteocalcin, testis morphometery, azoospermy, busulfan
     
Type of Study: Orginal Article | Subject: Anatomy
Received: 2017/06/23 | Accepted: 2018/12/30
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