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Anti-biofilm effect of peppermint extract on Pseudomonas aeruginosa that isolated of hospitalized patients in Tabriz city
Paria Vaghri , Mansoor Aletaha * 1
1- , manal239@yahoo.com
Abstract:   (129 Views)
IIntroduction
Biofilms may form on living or non-living surfaces and can be prevalent in natural, industrial and hospital settings. Pseudomonas aeruginosa is a pathogenic agent and has a high potential for biofilm formation. A study on herbal medicines to control biofilms is of particular importance. The aim of this study is to determine the effect of peppermint extract on bacterial and biofilm Pseudomonas aeruginosa isolated from clinical specimens and determining the frequency of the pelF gene and its association with biofilm formation.
Materials and Methods
208 clinical specimens were collected. The extract of peppermint was extracted and its effect on growth of Pseudomonas aeruginosa was evaluated and MIC and MBC of extract was determined. The appropriate time and intensity of biofilm formation as weak, moderate and strong was determined and the lowest concentration of biofilm formation inhibition (BIC) by peppermint extract was determined. Then the presence of pelF gene was tested by PCR.
Results
Peppermint extract caused formation of a non-growth halo on Pseudomonas aeruginosa bacteria. MIC and MBC concentrations of peppermint extract were determined to be 50 and 100 mg/ml respectively. The time for biofilm formation was 48 hours and biofilm inhibitory concentration (BIC) extract of peppermint was obtained at 50 mg/ml. One hundred percent of the isolated samples had PelF gene and this gene showed a significant relationship with biofilm formation (P=0.0001).
Conclusion
It is possible to use mint extract in various compositions to inhibit Pseudomonas aeruginosa and inhibit biofilm formation.
Keywords: Pseudomonas aeruginosa, Biofilm, Peppermint extract, Pel gene, PCR
     
Type of Study: Orginal Article | Subject: microbiology
Received: 2018/11/4 | Revised: 2019/05/19 | Accepted: 2019/02/2 | ePublished ahead of print: 2019/05/21
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