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:: Volume 7, Issue 2 (Vol.7, No.2,Summer 2019) ::
J Neyshabur Univ Med Sci 2019, 7(2): 23-33 Back to browse issues page
Evaluation of Protoscolex Antigens for Hydatidosis Detection before and after Surgery
Ali Jelowdar1 , Mahmood Safi1 , Abdollah Rafiei * 2, Molouk Beiromvand1 , Mahmood Rahdar1
1- Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2- Department of Parasitology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , rafieiabdollah@yahoo.com
Abstract:   (132 Views)
Introduction
Cystic echinococcosis (CE) is a zoonotic infection caused Echinococcus granulosus with worldwide distribution. As one of the problems that can be encountered after treating CE patients is the risk of postsurgical relapses or treatment failure, a long-term clinical and serological follow-up is required to evaluate the success and failure of therapy.
Materials and Methods
We used extract protoscolex antigen from fresh sheep hydatid cyst collect from slaughtered in Ahvaz. Overall we tested 180 serum samples comprising: 41 sera from post surgically confirmed CE from 6 month to 20 years after surgery, 69 sera from patients with symptomatic CE and 50 sera from non CE patients and 20 heterologous sera were evaluated with SDS-PAGE gel electrophoresis and western blotting.
Results
The sensitive of the 38 KDa PSC Antigen was 67 % pre and 83 % after surgery and 100% specify. All hepatic CE and 66.6 % of pulmonary CE sera was reacted with PSC Antigen.
Conclusion
The results indicate that the hydatid specific antibodies of IgG, IgG1 and IgG4 are the most important antibodies for the serological diagnosis of CE during the active stage of the disease and Ig G, particularly sub-class Ig G4 was more suitable for postoperative follow up.
Keywords: echinococcosis, Echinococcus granulosus, antigens, diagnosis
Full-Text [PDF 1080 kb]   (84 Downloads)    
Type of Study: Orginal Article | Subject: laboratory sciences
Received: 2019/03/17 | Revised: 2019/07/2 | Accepted: 2019/06/10 | ePublished ahead of print: 2019/07/2 | Published: 2019/07/6 | ePublished: 2019/07/6
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Jelowdar A, Safi M, Rafiei A, Beiromvand M, Rahdar M. Evaluation of Protoscolex Antigens for Hydatidosis Detection before and after Surgery. J Neyshabur Univ Med Sci. 2019; 7 (2) :23-33
URL: http://journal.nums.ac.ir/article-1-704-en.html


Volume 7, Issue 2 (Vol.7, No.2,Summer 2019) Back to browse issues page
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