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The comparison of the effect of an aerobic training period and ozone therapy on gene expression of Beclin-1, AMPK and mTOR of articular cartilage tissue in rats model of osteoarthritis
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Parvin Rezaee *  1 |
| 1- , parvin.farzanegi@gmail.com |
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Abstract: (629 Views) |
Introduction
The aim of the research was to investigate the effect of 8 weeks’ exercise training and ozone therapy on articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression in rat’s model of osteoarthritis (OA).
Materials and Methods
In this experimental study, 40 adult male rats 210±10 gr were randomly divided into 5 groups: 1) control (CN), 2) OA, 3) OA + ozone, 4) OA + exercise training and 5) OA + ozone + exercise training groups. The knee OA was induced by the surgical method in rats. The exercise training included running on a treadmill for 8 weeks, five times a week, and 35-45 minutes in an exercise session and with 18m per minute speed.
Rats in the ozone group received ozone (O3) at a concentration of 20μg/ml, once weekly for 3 weeks. 48 hours after the programs and fasting, cartilage tissues were isolated, and articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression were considered using Real-Time PCR (RT-PCR).
Results
In cartilage tissue, Beclin-1levels had a significant decrease in the OA group, but AMPK and mTOR levels increase. Ozone, exercise training, and ozone + exercise training Caused to reverse the process (P=0.009). But, there was no significant difference between these groups (P≥0.05).
Conclusion
According to the results of this study, ozone therapy and moderate-intensity exercise training Increase Beclin-1levels in the articular cartilage tissue. However, moderate-intensity exercise training with adjustable levels of mTOR may Play a greater role in the control of articular chondrocyte autophagy in rat’s model of OA. |
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| Keywords: Aerobic Exercise, Ozone Therapy, Osteoarthritis, Beclin-1, AMPK, mTOR |
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Full-Text [PDF 727 kb]
(33 Downloads)
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Type of Study: Orginal Article |
Subject:
Physiology
Received: 2019/08/26 | Revised: 2020/12/21 | Accepted: 2020/01/6 | ePublished ahead of print: 2020/06/7 | Published: 2020/05/30 | ePublished: 2020/05/30
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