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Prevalence of blaZ in Clinical Isolates of Staphylococcus aureus Isolated from Hamedan Hospitals
Romina Kakaei , AmirHossein Momen * 1, Hossein Vazini
1- , amir-hossein.momen1@uqac.ca
Abstract:   (145 Views)
Introduction
Staphylococcus aureus has been recognized as a powerful pathogen that causes numerous infections. Today, S.aureus has acquired multiple resistances to a wide range of antibiotics, including beta-lactams. Beta-lactamases hydrolyze beta-lactam antibiotics and are expressed through activation of the blaZ gene. The aim of this study was to evaluate the frequency of blaZ gene in clinical isolates of S.aureus isolated from Hamadan hospitals.
Materials and Methods
In this descriptive cross-sectional study, all S.aureus strains were randomly selected and transferred to the laboratory. In clinical isolates confirmed by biochemical tests of genomic DNA extraction procedures using Synagen Cat extraction kit. No.PR881614) was performed according to the manufacturer's protocol and 1% agarose gel was used to view the PCR product. Data were analyzed by SPSS software.
Results
The results of this study showed that 32 patients (64%) and 18 women (36%) were isolated from S.aureus hospitalized in Fatemieh and Sina hospitals. The pattern of antibiotic resistance obtained was that penicillin (10 µg) with 100% resistance and cefazolin (30 µg) with 5% resistance had the highest and lowest resistance, respectively. Also 94% of isolates were resistant to cefoxitin (30 µg) and 90% of isolates were resistant to oxacillin (1 µg). Of 50 S.aureus isolated from clinical isolates, 44 contained the blaZ beta-lactamase gene.
Conclusion
Based on the findings of this study, isolates isolated from clinical specimens showed the same pattern of abundanceTherefore, it is likely that more or more drug resistance will be transmitted to hospitals.
Keywords: Genes, Staphylococcus, Isolate, Drug resistance, Microbial
     
Type of Study: Orginal Article | Subject: microbiology
Received: 2019/11/2 | Revised: 2020/03/1 | Accepted: 2020/05/19 | ePublished ahead of print: 2020/06/17 | Published: 2020/05/30 | ePublished: 2020/05/30
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