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:: Volume 3, Issue 4 (Vol.3, No.4, Winter 2016) ::
J Neyshabur Univ Med Sci 2016, 3(4): 64-70 Back to browse issues page
The Survey of the Turbidity Interference Rate in 24-Hour Urine Protein Measurement before and after Cold Treatment in Centrifuged Samples
Masoud Mobini, Maryam Asghar Heydari, Mohammad Salehi *, Mahmud Gholami
Abstract:   (4008 Views)

Introduction and Aims
Proteinuria means the presence of an excess of serum proteins in the urine that is very important to achieve accurate and reliable results to the physicians. The aim of this study was to evaluate the interference of turbidity in the measurement of 24-hour urine protein before and after cold treatment in turbid centrifuged samples.
Materials and Methods
This experimental study was performed in ACECR (Academic Center for Education, Culture and Research), that was conducted on a random sample of 1000 people in 2015 March to September. The measurement of 24-hour urine protein was performed by using three chloral acetic acid method (TCA) and photometer.
Results
Ten samples (1%) just were turbid after centrifuge. There was a significant difference (P<0.05) between the samples which were turbid after centrifuge and the samples which their turbidity have removed by cold treatment. So that, in some cases the interpretation of the results after cold treatment is quite different with before (the lowest difference was 0.05 and most difference was 0.43).
Conclusion
In order to deposit interfering factors, the urine samples that are taken to measure protein and are turbid after centrifuge should be given cold treatment and centrifuge again.


* Corresponding Author: Iranian Academic Center for Education, Medical Diagnostic Laboratory of ACECR-Razavi Khorasan.
Email: Mohammadsalehi73@gmail.com

Keywords: proteinuria, urine turbid interference rate, 24 hour urine protein, treatment, cold temperature
Full-Text [PDF 1140 kb]   (1233 Downloads)    
Type of Study: Orginal Article |
Received: 2015/10/4 | Revised: 2018/09/3 | Accepted: 2016/01/28 | Published: 2016/03/5 | ePublished: 2016/03/5
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Mobini M, Asghar Heydari M, Salehi M, Gholami M. The Survey of the Turbidity Interference Rate in 24-Hour Urine Protein Measurement before and after Cold Treatment in Centrifuged Samples. J Neyshabur Univ Med Sci. 2016; 3 (4) :64-70
URL: http://journal.nums.ac.ir/article-1-209-en.html


Volume 3, Issue 4 (Vol.3, No.4, Winter 2016) Back to browse issues page
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