:: Volume 8, Issue 1 (Vol.8, No.1,Spring 2020) ::
J Neyshabur Univ Med Sci 2020, 8(1): 25-37 Back to browse issues page
Effect of Stachys setifera essential oil on human breast cancer MCF-7 cells line
Amir Shirzad1 , Akbar Safipour Afshar Dr. * 2, Fatemeh Saeid Nematpour Dr1
1- Department of Biology, Faculty of Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2- Department of Biology, Faculty of Sciences, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran , asafshar@iau-neyshabur.ac.ir
Abstract:   (776 Views)
Introduction
Breast cancer is the most common cancer among women. One of the approaches to prevent and treat various cancers is to use plant compounds. The aim of this study was to investigate the effect of S. setifera essential oil on MCF-7 human breast cancer cell line and comparing it with Doxorubicin as a known anti-cancer medicine.
Materials and Methods
Cells are treated by 0.01-0.025 µl/ml of essential oil at at 24, 48 and 72 hours and cell viability and colony formation ability were quantified. Also, essential oil compositin is analyzed by GC-Mass.
Results
The S. setifera essential oil decreased the viability and CFU of cells, significantly. EO IC50 was reported as 0.0206, 0.0175 and 0.0173 µl/ml over 24, 48 and 72 hours. In addition, the S. setifera essential oil GC-Mass analysis showed that most compounds are related to hydrocarbon alkanes and terpenoids.
Conclusion
Probably the essential oil cytotoxicity effects are related to hydrocarbon alkanes and terpenoids compounds.

Keywords: Stachys setifera, Breast Cancer, Cytotoxicity, MCF7, MTT assay.
Full-Text [PDF 1627 kb]   (150 Downloads)    
Type of Study: Orginal Article | Subject: biology
Received: 2019/08/12 | Revised: 2020/07/4 | Accepted: 2020/01/7 | Published: 2020/05/10 | ePublished: 2020/05/10


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Volume 8, Issue 1 (Vol.8, No.1,Spring 2020) Back to browse issues page