[Home ] [Archive]   [ فارسی ]  
:: Volume 8, Issue 4 (Vol.8, No.4,Winter 2020) ::
J Neyshabur Univ Med Sci 2020, 8(4): 124-135 Back to browse issues page
The comparison of the effect of an aerobic training period and ozone therapy on gene expression of Beclin-1, AMPK and mTOR of articular cartilage tissue in rats model of osteoarthritis
Parvin Rezaee * 1
1- , parvin.farzanegi@gmail.com
Abstract:   (594 Views)
Introduction
The aim of the research was to investigate the effect of 8 weeks’ exercise training and ozone therapy on articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression in rat’s model of osteoarthritis (OA).
Materials and Methods
In this experimental study, 40 adult male rats 210±10 gr were randomly divided into 5 groups: 1) control (CN), 2) OA, 3) OA + ozone, 4) OA + exercise training and 5) OA + ozone + exercise training groups. The knee OA was induced by the surgical method in rats. The exercise training included running on a treadmill for 8 weeks, five times a week, and 35-45 minutes in an exercise session and with 18m per minute speed.
Rats in the ozone group received ozone (O3) at a concentration of 20μg/ml, once weekly for 3 weeks. 48 hours after the programs and fasting, cartilage tissues were isolated, and articular cartilage tissue mRNA Beclin-1, AMPK, and mTOR gene expression were considered using Real-Time PCR (RT-PCR).
Results
In cartilage tissue, Beclin-1levels had a significant decrease in the OA group, but AMPK and mTOR levels increase. Ozone, exercise training, and ozone + exercise training Caused to reverse the process (P=0.009). But, there was no significant difference between these groups (P≥0.05).
Conclusion
According to the results of this study, ozone therapy and moderate-intensity exercise training Increase Beclin-1levels in the articular cartilage tissue. However, moderate-intensity exercise training with adjustable levels of mTOR may Play a greater role in the control of articular chondrocyte autophagy in rat’s model of OA.
Keywords: Aerobic Exercise, Ozone Therapy, Osteoarthritis, Beclin-1, AMPK, mTOR
Full-Text [PDF 727 kb]   (25 Downloads)    
Type of Study: Orginal Article | Subject: Physiology
Received: 2019/08/26 | Revised: 2020/12/21 | Accepted: 2020/01/6 | ePublished ahead of print: 2020/06/7 | Published: 2020/05/30 | ePublished: 2020/05/30
Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

rezaee P. The comparison of the effect of an aerobic training period and ozone therapy on gene expression of Beclin-1, AMPK and mTOR of articular cartilage tissue in rats model of osteoarthritis. J Neyshabur Univ Med Sci. 2020; 8 (4) :124-135
URL: http://journal.nums.ac.ir/article-1-801-en.html


Volume 8, Issue 4 (Vol.8, No.4,Winter 2020) Back to browse issues page
مجله علوم پزشکی نیشابور J Neyshabur Univ Med Sci

*** هرگونه کپی برداری از این سایت بدون ذکر منبع غیر قانونی و همراه با پیگیری های قضایی می باشد *** مجله دانشکده علوم پزشکی نیشابور *** Any copying of this site without citing the source is illegal And is associated with the pursuit of justice ***

Persian site map - English site map - Created in 0.07 seconds with 30 queries by YEKTAWEB 4250